The O'Sullivan lab takes a interdisciplinary approach to discover how to unleash the power of the innate immune system. Using systems biology, we discover novel layers of cellular regulation at the transcriptional, epigenetic and metabolic levels to activate or suppress the innate immune system during disease. We then test these mechanisms using preclinical mouse models to discover conserved and non-conserved pathways that can be either drugged or engineered to enhance immune function. To this end, we have pioneered several CRISPR editing methods in primary innate immune cells to discover new gene targets and model patient-specific mutations. We have focused on 3 major research areas: